By definition, intrinsic viscosityis “a measure of a solute’s contribution to the viscosity of a solution”, or more specifically an increase in viscosity as a result of adding an infinitesimal amount of solute to a solvent. Intrinsic viscosity is an important tool in the fields of polymer and protein research. Intrinsic viscosity allows for determination of molecular weight and size more reliably than the traditional method for determining these characteristics - dynamic light scattering.
To determine the intrinsic viscosity, you must measure the viscosity of your solution at different concentrations and then apply theHuggins and Kraemer equation. The intrinsic viscosity is a reliable and sensitive indicator of a solute’s molecular interaction with a solvent, and can be used to determine the molecular properties of a sample, such as:
Molecular weight and size
Interaction of molecules
Stability of molecules: aggregation, denaturation, or conformational changes of protein molecules
Protein structure and melting temperatures
Industries Where Intrinsic Viscosity is Important:
Polymers/Coatings (R&D, QC)
Food Polymers (Hydrodynamic Volume of Biopolymers)
The m-VROC® small sample viscometer is an ideal tool to measure intrinsic viscosity of samples. The high accuracy, low sample volume requirements, and high speed of measurement ensure that data collection is fast, simple, and accurate. Advantages of using the m-VROC over conventional viscometers including:
Smallest Sample Volume
Temperature Control: 4 - 70ºC
Superior Accuracy (2% of Reading) and Repeatability (0.5% of Reading)
No Air-Liquid Interface (No Evaporation or Contamination)
Wide Dynamic Range:
Viscosity: 0.2- 200,000 [cP]
Shear Rate: 0.5 - 1,400,000 [1/s]
To learn more about how you can use our m-VROC to measure intrinsic viscosity, download our application note "Intrinsic Viscosity with Repeatable, Small Sample Viscometer, m-VROC"
Want to learn more about calculating intrinsic viscosity and how to apply it to your molecular characterizations? Download our recorded webinar: "Intrinsic Viscosity — A Tool to Determine Molecular Size & Structure Through Viscosity Measurements" This webinar presents analytical and experimental procedures necessary to establish intrinsic viscosity. We also present a number of examples on how this property can be useful for various polymer and biopolymer applications.