Measuring viscosity dates back to as early as the 19th century. French physicist Jean Poiseuille discovered the concept of measuring viscosity by formulating the "mathematical expression for the flow rate for the laminar flow of fluids in circular tubes." Later on, this formulation was discovered by a German hydraulic engineer Gotthilf Hagen, which came to be known as the Hagen-Poiseuille equation (Britannica). Early measurements of viscosity focused primarily on the flow of blood. Measurements were conducted using the hemodynamometer that incorporated narrow tubes & glass capillaries in effort to measure the pressures in the arteries of horses and dogs (Sutera).
This month, one of our partners in Korea, Insung Chromatech, hosted a seminar in Korea's Science Town. Science Town is a well known area in South Korea for the research & development hub of leading institutes and companies.
One of the challenges of creating monoclonal antibody (mAb) injectable drugs is optimizing formulation. A good formulation has to have a high concentration of stable mAb’s for high efficacy. There are a variety of factors that can affect stability of these mAb’s, temperature, shear forces, pH, and even concentration. When proteins become, unstable and denature they can increase the viscosity of your formulation. Using a simple modified parallel plate demonstration, we can visualize why denatured proteins increase viscosity.
Do you subconsciously dread the first drop of ketchup out of its bottle? Have you noticed the inconsistent flow in the substance as you try to coax the ketchup out, only to have a huge gush of flow when you realize you have coaxed it a little too well?
SAN RAMON, CA – May 14, 2016 – RheoSense, Inc. is proud to introduce VROC® initium, the first automatic viscometer that revolutionizes viscosity characterization. Equipped with automatic sample loading and sample cleaning, VROC® initium measures absolute viscosity as a function of shear rate across a wide temperature range. This allows unique viscosity fingerprinting of your samples. Samples in 96 well plate as well as 40 vial racks can be tested automatically with our intuitive software.
The exact mechanism by which Arginine molecules affect protein molecules in solution is unclear. Nonetheless, its benefits increasing solubility and controlling viscosity have spread out its application in biopharma formulations, specially for high concentration antibody solutions.
In our application note, we examine the effect of concentration on the viscosity of dilute solutions of L-Arginine in water and PBS. Additionally, we study the Intrinsic Viscosity of this solutions to better understand molecular size and solubility in dilution.